Ez-fit enzyme kinetics program

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Thapa HB. Growth of five fast growing tree species in the Terai of eastern Nepal. Banko Janakari. Rajendra KC. Olsen CS, Helles F. Medicinal plants, markets, and margins in the Nepal Himalaya: trouble in paradise. Mt Res Dev. Several different graphs can be displayed: an X-Y, a Scatchard, an Eadie-Hofstee, a Lineweaver-Burk, a semilogarithmic, and a residual plot. A data analysis report and graphs are designed to evaluate the goodness-of-fit of the data to a particular model.

Enter data in the worksheet. Fill first X column with data of the first independent variable. If multiple measurements for the first independent variable are defined, multiple X columns need to be filled. If there are 2 independent variables, specify the value for the second independent variable of each group of Y data in its column header Indep2 Value. Fill each Y column with data for the dependent variable.

Click the upper-left button Fit Model in the worksheet, to open the dialog for fitting. Choose the reaction and the study type. If there are 2 independent variables, choose 1st Independent Variable for the fitting function from the drop-down list, make sure the 1st Independent variable here must be consistent with the worksheet's data settings, and 2nd Independent Variable will be updated automatically in the dialog.

A report sheet and a report data sheet will be created which include fitted results and the ranking models result. A library of 46 kinetic models is included to facilitate the analysis of enzyme kinetic data, or you can create your own custom User-Defined-Functions UDF for curve-fitting. A UDF can have up to 8 parameters P P8 and 3 independent X X3 variables. BestCurvFit saves the results of each analysis in a text file with the data file name.

It also creates a dated log file of all analyses which you should delete if it becomes too large. Rather it's a stand alone executable program with no dynamic link library files DLL's. BestCurvFit Accuracy. NIST states that "a good nonlinear least squares procedure should be able to duplicate the certified results to at least 4 or 5 digits".

BestCurvFit analyses of 22 NIST datasets produced an average accuracy of more than 6 decimal places using numerical derivatives. See the FAQ page for further details.

The BestCurvFit software is copy protected to run on one computer only. One additional license is allowed upon request as a backup if your computer is replaced. Simply download the software and run BestCurvFit on the computer you want to license it on and email us DrFrank88 gmail.

There are no time limits or number of uses once the software is activated. See the Software page for further details. Reaction scheme of the General Modifier Mechanism.

This scheme is based on the rapid-equilibrium assumption and describes the basic mechanism for the all classical reversible inhibition mechanisms. At saturating inhibitor concentrations the enzyme activity is either fully or partially inhibited depending on the value of 'b'. In other words, at saturating inhibitor concentrations the remaining enzyme activity is either fully or partially decreased, respectively.

The factor 'a' represents the difference in binding affinities between the substrate binding to the EI complex and the inhibitor binding to the ES complex. Differing values of 'a' and 'b' can be used to obtain all the common types of inhibition models. Competitive, uncompetitive, noncompetitive, or mixed type inhibition can generally be distinguished by the values of 'a' and Ki.

Scheme and Equation for the General Modifier Mechanism. E: enzyme, ES: enzyme-substrate complex, ESI: enzyme-substrate-inhibitor complex, S: substrate, I: inhibitor, P: product of reaction, Km: substrate dissociation constant, Ki: inhibitor binding dissociation constant, kcat: catalytic rate constant, and 'a' and 'b' are dimensionless constants that define the type of the inhibition mechanism.

See link to Antonio Baici's Website below:. Example of Yonetani-Theorell model. Yonetani-Theorell method of exclusivity of inhibitor interactions with an enzyme involves measuring the initial velocity of the enzyme at different concentrations of two inhibitors.

BestCurvFit plot of Yonetani-Theorell analysis.